Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h)
Identifieur interne : 000753 ( Main/Exploration ); précédent : 000752; suivant : 000754Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h)
Auteurs : Zi-Shu Chen ; Ning-Ning Han ; Jian-Hong Li ; Guo-Hua Huang ; Hu WanSource :
- Scientific Reports [ 2045-2322 ] ; 2017.
Abstract
Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp
Url:
DOI: 10.1038/s41598-017-07684-w
PubMed: 28765578
PubMed Central: 5539149
Affiliations:
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China</wicri:noCountry></affiliation></author><author><name sortKey="Li, Jian Hong" sort="Li, Jian Hong" uniqKey="Li J" first="Jian-Hong" last="Li">Jian-Hong Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Huang, Guo Hua" sort="Huang, Guo Hua" uniqKey="Huang G" first="Guo-Hua" last="Huang">Guo-Hua Huang</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.257160.7</institution-id><institution>Institute of 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type="wicri:Area/Main/Exploration">000753</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of <italic>Heliothis virescens</italic> ascovirus 3 h (HvAV-3h)</title><author><name sortKey="Chen, Zi Shu" sort="Chen, Zi Shu" uniqKey="Chen Z" first="Zi-Shu" last="Chen">Zi-Shu Chen</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.257160.7</institution-id><institution>Institute of Virology, College of Plant protection,</institution><institution>Hunan Agricultural University,</institution></institution-wrap>Changsha, 410128 Hunan China</nlm:aff><wicri:noCountry code="subfield">410128 Hunan China</wicri:noCountry></affiliation></author><author><name sortKey="Han, Ning Ning" sort="Han, Ning Ning" uniqKey="Han N" first="Ning-Ning" last="Han">Ning-Ning Han</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Li, Jian Hong" sort="Li, Jian Hong" uniqKey="Li J" first="Jian-Hong" last="Li">Jian-Hong Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Huang, Guo Hua" sort="Huang, Guo Hua" uniqKey="Huang G" first="Guo-Hua" last="Huang">Guo-Hua Huang</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.257160.7</institution-id><institution>Institute of Virology, College of Plant protection,</institution><institution>Hunan Agricultural University,</institution></institution-wrap>Changsha, 410128 Hunan China</nlm:aff><wicri:noCountry code="subfield">410128 Hunan China</wicri:noCountry></affiliation></author><author><name sortKey="Wan, Hu" sort="Wan, Hu" uniqKey="Wan H" first="Hu" last="Wan">Hu Wan</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1790 4137</institution-id><institution-id institution-id-type="GRID">grid.35155.37</institution-id><institution>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology,</institution><institution>Huazhong Agricultural University,</institution></institution-wrap>Wuhan, 430070 Hubei China</nlm:aff><wicri:noCountry code="subfield">430070 Hubei China</wicri:noCountry></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp <italic>Microplitis similis</italic> Lyle. Ascovirus-parasitoids wasp-noctuid insects constitute the dissemination system. Selection of suitable reference genes for the dissemination system could play an important role in elucidating the pathogenic molecular mechanisms of ascovirus. Unfortunately, such studies on potential reference genes in the dissemination system of ascoviruses are lacking. In the present study, we evaluated 11 candidate reference genes: <italic>β-actin1</italic> (<italic>ACT1</italic>), <italic>β-actin2</italic> (<italic>ACT2</italic>), <italic>elongation factor 1</italic> (<italic>EF1</italic>), <italic>elongation factor 2</italic> (<italic>EF2</italic>), <italic>ribosomal protein L10</italic> (<italic>L10</italic>), <italic>ribosomal protein L17A</italic> (<italic>L17A</italic>), <italic>superoxide dismutase</italic> (<italic>SOD</italic>), <italic>28S ribosome</italic> (<italic>28S</italic>), <italic>Tubulin</italic> (<italic>TUB</italic>) and <italic>18S ribosome</italic> (<italic>18S</italic>). The samples were originally from various virus concentrations and points-in-time of experimental treatments using RefFinder and four algorithms. The results showed that <italic>EF1</italic> was the most stable internal gene in <italic>S. exigua</italic> and <italic>M. similis</italic> and that <italic>EF2</italic> was the most stable in the IOZCAS-Spex-II-A cell line, and the stability of reference genes were confirmed via the expression levels of two inhibitor of apoptosis-like (<italic>iap</italic>-like) genes from <italic>Heliothis virescens</italic> ascovirus 3 h (HvAV-3h). This study provides a crucial basis for future research that explores the molecular mechanisms of the pathogenesis of ascoviruses.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Stasiak, K" uniqKey="Stasiak K">K Stasiak</name></author><author><name sortKey="Renault, S" uniqKey="Renault S">S Renault</name></author><author><name sortKey="Demattei, Mv" uniqKey="Demattei M">MV Demattei</name></author><author><name sortKey="Bigot, Y" uniqKey="Bigot Y">Y Bigot</name></author><author><name sortKey="Federici, Ba" uniqKey="Federici B">BA Federici</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Asgari, S" uniqKey="Asgari S">S Asgari</name></author><author><name sortKey="Davis, J" uniqKey="Davis J">J Davis</name></author><author><name sortKey="Wood, D" uniqKey="Wood D">D Wood</name></author><author><name sortKey="Wilson, P" uniqKey="Wilson P">P Wilson</name></author><author><name sortKey="Mcgrath, A" 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last="Han">Ning-Ning Han</name><name sortKey="Huang, Guo Hua" sort="Huang, Guo Hua" uniqKey="Huang G" first="Guo-Hua" last="Huang">Guo-Hua Huang</name><name sortKey="Li, Jian Hong" sort="Li, Jian Hong" uniqKey="Li J" first="Jian-Hong" last="Li">Jian-Hong Li</name><name sortKey="Wan, Hu" sort="Wan, Hu" uniqKey="Wan H" first="Hu" last="Wan">Hu Wan</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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